Transformation-derived Neisseria gonorrhoeae plasmids with altered structure and function

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Conjugative Plasmids of Neisseria gonorrhoeae

Many clinical isolates of the human pathogen Neisseria gonorrhoeae contain conjugative plasmids. The host range of these plasmids is limited to Neisseria species, but presence of a tetracycline (tetM) determinant inserted in several of these plasmids is an important cause of the rapid spread of tetracycline resistance. Previously plasmids with different backbones (Dutch and American type backbo...

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Conjugative plasmids in Neisseria gonorrhoeae.

A conjugation system initially discovered in beta-lactamase-producing gonococci mobilized small non-selftransmissible R plasmids encoding beta-lactamase (penicillinase) production into other gonococci, Neisseria, and Escherichia coli. This conjugation system was mediated by a separate selftransmissible plasmid of 23.9 X 10(6) daltons, pFA2. Conjugative plasmids capable of mobilizing R plasmids ...

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Plasmids of Neisseria gonorrhoeae and other Neisseria species.

a These species were shown to carry plasmids of various sizes which hybridized with probes made from the 2.6-MDa plasmid. b Related plasmids are found in the genus Haemophilus (4). Identical plasmids are found in clinical isolates of K. denitrificans and Eikenella corrodens (24). d Related plasmids are found in enteric bacteria and Eikenella corrodens

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Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

DNA probe hybridisation was used to examine the relation between the cryptic plasmid from Neisseria gonorrhoeae and plasmids carried by pharyngeal isolates of Neisseria meningitidis and Neisseria lactamica. The complete gonococcal cryptic plasmid and HinfI derived digestion fragments subcloned into Escherichia coli were used to probe Southern blots of plasmid extracts. Homology was found to a p...

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Factors affecting genetic transformation of Neisseria gonorrhoeae.

Piliated gonococci were competent in genetic transformation in all stages of growth in minimal and enriched media, but nonpiliated cells were almost totally incompetent. Uptake of deoxyribonucleic acid into a deoxyribonuclease-insensitive state was observed only in competent piliated cells. Competence was not affected by washing of competent cells or treatment of competent cells with proteolyti...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1979

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.138.2.510-518.1979